Fluorescence Correlation Spectroscopy Single Molecule Analyzer CX Series

The module can be combined with a wide field, confocal, total internal reflection, multiphoton, light sheet, and STED super-resolution microscopy platforms to integrate fluorescence spectroscopy with single molecule resolution into the advantages of each fluorescence microscopy imaging mode. Detection of molecular molar concentration and interaction affinity (Kd value) in living cells Quantitative analysis of molecular and nanoparticle properties in cell lysates or solution samples The world's first commercial single-molecule fluorescence research instrument

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Product description

Parameters

Versatile Analysis of Biomacromolecules, Nanoparticle Properties in Single Live Cells or solution samples:

Molecular interaction affinity and kinetic constants
Single molecule conformation and conformational conversion rate
Diffusion coefficient/hydrodynamic radius of the molecule or nanoparticle
Molarity of the molecule or nanoparticle

Examples of Cutting-edge Applications:

Liquid-liquid phase separation of biological macromolecules
Molecular interactions in cell biology and drug screening
Molecular dynamic structure-functional mechanisms
Photophysical properties of fluorescent probe molecules or nanoparticles
Denatured aggregation of biological macromolecules
Stability and metabolic analysis of nanodrugs in plasma

Product Characteristics

The module can be combined with multiple microscope platforms such as widefield, confocal and total internal reflection, multiphoton, optical sheet, and STED super-resolution to integrate single-molecule resolution fluorescence spectroscopy with the advantages of each fluorescence microscopy imaging mode.

Functional Indicators

Linear range for measuring molecular concentration: 10pM-100nM

Measure the hydrodynamic radius of a molecule or nanoparticle: <300 nm

Measure the dissociation constant of molecular binding (e.g., antigen-antibody) reactions K_D Measurement range: pM-μM

Analysis of single-molecule short fluorescence signals: such as Alternative Laser Excitaion Single-molecule FRET

Typical User

Guangdong Songshan Lake Materials Laboratory

Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences

National and local joint engineering laboratory for animal peptide drug creation, Hunan Normal University

Model Configuration

Model Configuration Table
●Standard ○Optional X Not Optional ☆Customized
Model			CX100	CX200	CX300
Function	Fluorescence Autocorrelation Analysis		●	●	●
	Fluorescence Cross-Correlation Analysis		○	●	●
	Single Molecule Fluorescence Analysis		X	○	●
Laser	Laser I.		●	●	●
	Laser II.		○	●	●
	Laser III.		○	○	●
	Alternately Excited		X	○	●
Detector	Detector I.		●	●	●
Detector	Detector II		○	●	●
Optical System	High-Medium Digital Aperture Apochromatic Water Mirror		●	●	●
Optical System	Confocal Pinhole Automatic Calibration System		●	●	●
Correlator Cards	Hardware Correlator		●	●	●
Correlator Cards	Sequence of Single-Photon Signals Output		X	○	●
Sample Storage	Laser Protective Device		●	●	●
	Termometer		●	●	●
	Temperature Controlling Equipment		○	○	●
	Electric Sample Stage		●	●	●
	Eight-Well Box Sample Cell		X	X	X
	Customization Sample Cell		☆	☆	☆
Others	Microscope Interface		☆	☆	☆
Software	Equipment Control and Data Acquisition Software		●	●	●
	Data Analysis Software		●	●	●
	Extend Analytics Functions	Cross Correlation Analysis	○	●	●
		Single Molecule Function	X	○	●
		Molecular Interactions	●	●	●
		Particle Size Distribution	●	●	●
		Phase Change Analysis	X	○	○
		Exosome Analysis	X	○	○
	Data Analysis Functions Customization		☆	☆	☆

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