• Categories:FCS technology for nanoparticle analysis
• Time of issue:2023-05-24 14:56
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(Summary description)Kaloian Koynov's group at the Max Planck Institute for Polymer Research, University of Mainz, Germany, has developed a fluorescence correlation spectroscopy (FCS)-based technique for the precise characterization of fluorescently labeled nano-drug carriers (NCs) in less than 50 μL of whole blood samples. size, concentration and drug loading efficiency. Due to the strong absorption of light by blood in the visible range and the large size of blood cells that completely occupy the FCS detection volume, the detection of fluorescent signals is hindered. Therefore, the plasma separation membrane is used as the separation medium in the whole blood sample, so that the liquid part and NCs in the blood can pass through the detection volume, while preventing the entry of larger blood cells, thus realizing the monitoring of the "fate" of NCs in blood. In this paper, the FCS data of Alexa 647 and NCs in plasma, whole blood (through plasma separation membrane) and PBS were first collected, and then NCs were injected into mice, and samples were taken at specific time intervals. The results showed that the autocorrelation curves detected in conventional plasma and whole blood were basically consistent, indicating that the newly developed blood drop method FCS can be successfully used to monitor and characterize NCs in blood. Applying the above methods will provide new ideas for monitoring the integrity of NCs in the blood circulation, thereby facilitating the development of NCs with transient stability and predictable biodegradability.