• Categories:FCS technology for nanoparticle analysis
• Time of issue:2023-05-24 14:50
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(Summary description)The team of Hans-Jürgen Butt and Kaloian Koynov at the Max Planck Institute for Polymer Research in Germany reported the quantitative analysis of nanomedicine carriers (NCs) in human blood using near-infrared fluorescence correlation spectroscopy (NIR-FCS). NCs have a unique role in the treatment of various diseases, and they may undergo changes such as aggregation and interaction in human blood, so it is necessary to directly detect NCs in blood. FCS technology can monitor the interaction of molecules in solution, particle size changes, etc., but blood can strongly absorb and scatter visible light, so reports of traditional FCS in blood experiments are rare. This paper introduces the method of NIR-FCS experiment in detail, including the determination of near-infrared fluorescent molecular diffusion coefficient, confocal volume, etc. In addition, this article avoids the influence of blood cells in the blood by collecting FCS data in the flowing blood and using a specific mathematical model, so that the FCS experiment can be performed without separating blood cells and plasma. Through the above method, the team further explored the possibility of NIR-FCS quantitative analysis of the interaction of small molecules in blood and the stability of drug carriers.